Review



myc parkin addgene  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    Addgene inc myc parkin addgene
    Myc Parkin Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/lentiCRISPRv2+puro+(Plasmid+%2398290)/pm41932308-260-45-46
    Average 98 stars, based on 523 article reviews
    myc parkin addgene - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    other:

    Article Title: The HIF-2 Transcription Factor Mediates Resistance to Ferroptosis in Pancreatic Cancer
    Article Snippet: Myc-Parkin , Addgene , Cat# 17612.

    Plasmid Preparation:

    Article Title: Deciphering functional roles and interplay between Beclin1 and Beclin2 in autophagosome formation and mitophagy.
    Article Snippet: The degradation of macromolecules and organelles by the process of autophagy is critical for cellular homeostasis and is often compromised during aging and disease.. Beclin1 and Beclin2 are implicated in autophagy induction, and these homologs share a high degree of amino acid sequence similarity but have divergent Nterminal regions.. Here, we investigated the functions of the Beclin homologs in regulating autophagy and mitophagy, a specialized form of autophagy that targets mitochondria.

    Article Title: Deciphering Functional Roles and Interplay Between Beclin1 and Beclin2 in Autophagosome Formation and Mitophagy
    Article Snippet: .. Plasmid Constructs GFP-LC3 (plasmid 11546), Myc-Parkin (plasmid 17612), mCherry-Parkin (plasmid #23956), pcDNA4-Beclin1-HA (plasmid #24399) ( 48 ), GFP-p40PX (plasmid #19010) and mCherry-DFCP1 (plasmid 86746) were obtained from Addgene. .. HA-Beclin1 S15A, HA-Beclin1 S15D, HA-Beclin1 S30A, HA-Beclin1 T57A, HA-Beclin1 S90A, HA-Beclin1 S93A mutants and HA-Beclin2 constructs were synthesized by Genscript using pcDNA3.1(+) as a backbone.

    Article Title: The Roles of Beclin1 and Beclin2 in Autophagy and Mitophagy
    Article Snippet: .. 19 Plasmids: GFP-LC3 (Addgene plasmid 11546), Myc-Parkin (Addgene plasmid 17612), a gift from Ted Dawon, mCherryParkin (Addgene plasmid 23956) was a gift from Richard Youle, pcDNA4-Beclin1-HA, a gift from Qing Zhong (Addgene plasmid 24399) (Sun, 2008). .. All custom constructs were designed by the author and generated by Genscript on the pcDNA3.1(+) backbone.

    Article Title: Isolation of Rab5-positive endosomes reveals a new mitochondrial degradation pathway utilized by BNIP3 and Parkin
    Article Snippet: .. Cells were transiently transfected with DNA using FuGENE 6 Transfection Reagent (Promega, E2691) according to the manufacturer's instructions with myc-Parkin (gift from Ted Dawson, Addgene plasmid #17612) and GFP-Rab5Q79L (gift from Qing Zhong, Addgene plasmid #28046). .. For imaging of mitochondria in BNIP3 experiments, cells were transfected with myc vector or myc-BNIP3 33 plus GFP-Rab5Q79L and cultured in the presence of 50 μM ZVAD (Millipore, 627610) to inhibit apoptosis.

    Article Title: Deciphering Functional Roles and Interplay Between Beclin1 and Beclin2 in Autophagosome Formation and Mitophagy
    Article Snippet: .. GFP-LC3 (plasmid 11546), Myc-Parkin (plasmid 17612), mCherry-Parkin (plasmid #23956), pcDNA4-Beclin1-HA (plasmid #24399) ( 48 ), GFP-p40PX (plasmid #19010) and mCherry-DFCP1 (plasmid 86746) were obtained from Addgene. .. HA-Beclin1 S15A, HA-Beclin1 S15D, HA-Beclin1 S30A, HA-Beclin1 T57A, HA-Beclin1 S90A, HA-Beclin1 S93A mutants and HA-Beclin2 constructs were synthesized by Genscript using pcDNA3.1(+) as a backbone.

    Article Title: Isolation of Rab5-positive endosomes reveals a new mitochondrial degradation pathway utilized by BNIP3 and Parkin
    Article Snippet: .. 4 Immunofluorescence Cells were transiently transfected with DNA using FuGENE 6 Transfection Reagent (Promega, E2691) according to the manufacturer's instructions with myc-Parkin (gift from Ted Dawson, Addgene plasmid #17612) and GFP-Rab5Q79L (gift from Qing Zhong, Addgene plasmid #28046). .. For imaging of mitochondria in BNIP3 experiments, cells were transfected with myc vector or myc-BNIP3 33 plus GFP-Rab5Q79L and cultured in the presence of 50 μM ZVAD (Millipore, 627610) to inhibit apoptosis.

    Generated:

    Article Title: Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cells
    Article Snippet: After 4 h, the medium was changed and the cells were incubated with fresh medium for 48 h. For plasmid transfection, cells were transfected with 1 μg of plasmid using TurboFect Transfection Reagent (Thermo Fisher Scientific, R0534) according to the manufacturer’s instructions. .. The plasmids were as follows: pmRFP-GFP encoding tandem-fluorescent LC3 (mRFP-GFP-LC3; a gift from Dr. Inhee Mook-Jung, Seoul National University); pmRFP-LC3 (Addgene, #21075); pDsRed2-Mito (Clontech, #632421); pEGFP-DFCP1 (Addgene, #38269); pEGFP-Parkin was generated by subcloning Myc-Parkin from pRK5-Myc-Parkin (Addgene, #17612; Ted Dawson’s lab) into pEGFP-C1 (Clontech, #6084-1). .. Cells were grown on coverslips coated with poly-L-lysine (Sigma, P4707), fixed in 10% formalin for 10 min and permeabilized with 0.1% saponin (Sigma, 47036) in PBS for 15 min. After washing with PBS, the coverslips were incubated with primary antibody diluted with antibody diluent solution (Invitrogen, #00-3118) for 1 h at room temperature (RT) and washed with PBS.

    Subcloning:

    Article Title: Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cells
    Article Snippet: After 4 h, the medium was changed and the cells were incubated with fresh medium for 48 h. For plasmid transfection, cells were transfected with 1 μg of plasmid using TurboFect Transfection Reagent (Thermo Fisher Scientific, R0534) according to the manufacturer’s instructions. .. The plasmids were as follows: pmRFP-GFP encoding tandem-fluorescent LC3 (mRFP-GFP-LC3; a gift from Dr. Inhee Mook-Jung, Seoul National University); pmRFP-LC3 (Addgene, #21075); pDsRed2-Mito (Clontech, #632421); pEGFP-DFCP1 (Addgene, #38269); pEGFP-Parkin was generated by subcloning Myc-Parkin from pRK5-Myc-Parkin (Addgene, #17612; Ted Dawson’s lab) into pEGFP-C1 (Clontech, #6084-1). .. Cells were grown on coverslips coated with poly-L-lysine (Sigma, P4707), fixed in 10% formalin for 10 min and permeabilized with 0.1% saponin (Sigma, 47036) in PBS for 15 min. After washing with PBS, the coverslips were incubated with primary antibody diluted with antibody diluent solution (Invitrogen, #00-3118) for 1 h at room temperature (RT) and washed with PBS.

    Construct:

    Article Title: Deciphering Functional Roles and Interplay Between Beclin1 and Beclin2 in Autophagosome Formation and Mitophagy
    Article Snippet: .. Plasmid Constructs GFP-LC3 (plasmid 11546), Myc-Parkin (plasmid 17612), mCherry-Parkin (plasmid #23956), pcDNA4-Beclin1-HA (plasmid #24399) ( 48 ), GFP-p40PX (plasmid #19010) and mCherry-DFCP1 (plasmid 86746) were obtained from Addgene. .. HA-Beclin1 S15A, HA-Beclin1 S15D, HA-Beclin1 S30A, HA-Beclin1 T57A, HA-Beclin1 S90A, HA-Beclin1 S93A mutants and HA-Beclin2 constructs were synthesized by Genscript using pcDNA3.1(+) as a backbone.

    Transfection:

    Article Title: Isolation of Rab5-positive endosomes reveals a new mitochondrial degradation pathway utilized by BNIP3 and Parkin
    Article Snippet: .. Cells were transiently transfected with DNA using FuGENE 6 Transfection Reagent (Promega, E2691) according to the manufacturer's instructions with myc-Parkin (gift from Ted Dawson, Addgene plasmid #17612) and GFP-Rab5Q79L (gift from Qing Zhong, Addgene plasmid #28046). .. For imaging of mitochondria in BNIP3 experiments, cells were transfected with myc vector or myc-BNIP3 33 plus GFP-Rab5Q79L and cultured in the presence of 50 μM ZVAD (Millipore, 627610) to inhibit apoptosis.

    Article Title: Isolation of Rab5-positive endosomes reveals a new mitochondrial degradation pathway utilized by BNIP3 and Parkin
    Article Snippet: .. 4 Immunofluorescence Cells were transiently transfected with DNA using FuGENE 6 Transfection Reagent (Promega, E2691) according to the manufacturer's instructions with myc-Parkin (gift from Ted Dawson, Addgene plasmid #17612) and GFP-Rab5Q79L (gift from Qing Zhong, Addgene plasmid #28046). .. For imaging of mitochondria in BNIP3 experiments, cells were transfected with myc vector or myc-BNIP3 33 plus GFP-Rab5Q79L and cultured in the presence of 50 μM ZVAD (Millipore, 627610) to inhibit apoptosis.

    Immunofluorescence:

    Article Title: Isolation of Rab5-positive endosomes reveals a new mitochondrial degradation pathway utilized by BNIP3 and Parkin
    Article Snippet: .. 4 Immunofluorescence Cells were transiently transfected with DNA using FuGENE 6 Transfection Reagent (Promega, E2691) according to the manufacturer's instructions with myc-Parkin (gift from Ted Dawson, Addgene plasmid #17612) and GFP-Rab5Q79L (gift from Qing Zhong, Addgene plasmid #28046). .. For imaging of mitochondria in BNIP3 experiments, cells were transfected with myc vector or myc-BNIP3 33 plus GFP-Rab5Q79L and cultured in the presence of 50 μM ZVAD (Millipore, 627610) to inhibit apoptosis.



    Similar Products

    98
    Addgene inc myc parkin addgene
    Myc Parkin Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/lentiCRISPRv2+puro+(Plasmid+%2398290)/pm41932308-260-45-46
    Average 98 stars, based on 1 article reviews
    myc parkin addgene - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    93
    Addgene inc myc parkin
    Myc Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/pRK5-Myc-Parkin+(Plasmid+%2317612)/pmc13052133-700-0-2
    Average 93 stars, based on 1 article reviews
    myc parkin - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc c myc tagged parkin
    C Myc Tagged Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/pRK5-Myc-Parkin+(Plasmid+%2317612)/pmc12852562-78-12-17
    Average 93 stars, based on 1 article reviews
    c myc tagged parkin - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc lck mturquoise2 ilid
    Lck Mturquoise2 Ilid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/pRK5-Myc-Parkin+(Plasmid+%2317612)/pm41387450-283-12-13
    Average 93 stars, based on 1 article reviews
    lck mturquoise2 ilid - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc plv sspbhalotag p63dh
    Plv Sspbhalotag P63dh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/pRK5-Myc-Parkin+(Plasmid+%2317612)/pm41387450-283-9-10
    Average 93 stars, based on 1 article reviews
    plv sspbhalotag p63dh - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc plv sspb halotag p63dh
    Plv Sspb Halotag P63dh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/pRK5-Myc-Parkin+(Plasmid+%2317612)/pmc12796338-355-9-10
    Average 93 stars, based on 1 article reviews
    plv sspb halotag p63dh - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc prk5 myc parkin
    Prk5 Myc Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+parkin/pRK5-Myc-Parkin+(Plasmid+%2317612)/pmc12507004-420-12-19
    Average 93 stars, based on 1 article reviews
    prk5 myc parkin - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results